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| Registros recuperados : 49 | |
| 5. |  | BASSO, M. F.; FAJARDO, T. V. M.; EIRAS, M.; AYUB, R. A.; NICKEL, O. Detecção e identificação molecular de vírus associados a videiras sintomáticas e assintomáticas. Ciência Rural, Santa Maria, v. 40, n. 11, p. 2249-2255, 2010.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 6. | | BASSO, M. F.; FAJARDO, T. V. M.; EIRAS, M.; BOTTON, M.; AYUB, R. A.; NICKEL, O. RT-PCR para detecção de vírus em vinhedos antigos e em cochonilhas vetoras. Tropical Plant Pathology, v. 35, p. S165, ago. 2010. Suplemento. Resumo (05.012) apresentado no XLIII Congresso Brasileiro de Fitopatologia, 2010, Cuiabá.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 8. | | BASSO, M. F.; FAJARDO, T. V. M.; EIRAS, M.; AYUB, R. A.; NICKEL, O. Produção de antissoro policlonal utilizando a proteína capsidial recombinante do Rupestris stem pitting-associated virus. Ciência Rural, Santa Maria, v. 40, n. 11, p. 2385-2388, 2010. Nota técnica.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 9. | | BASSO, M. F.; FAJARDO, T. V. M.; EIRAS, M.; AYUB, R. A.; NICKEL, O. Production of polyclonal antiserum using recombinant coat protein of Rupestris stem pitting-associated virus. Tropical Plant Pathology, v. 35, p. S272, ago. 2010. Suplemento. Resumo (11.013) apresentado no XLIII Congresso Brasileiro de Fitopatologia, 2010, Cuiabá.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 10. | | ARAÚJO, G. L. T.; BASSO, M. F.; MORGANTE, C. V.; SA, M. F. G. de. Overexpression of the GmGlb1-1 and GmEXPA-1 applied in cotton to increase tolerance to Meloidogyne incognita. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. p. 611.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 12. | | FAJARDO, T. V. M.; BASSO, M. F.; GUERRA, C. C.; SANTOS, H. P. dos; AYUB, R. A.; NICKEL, O. Alterações fisiolágicas e de qualidade da uva produzida em videiras infectadas por vírus. Tropical Plant Pathology, v. 35, p. S191, ago. 2010. Suplemento. Resumo (06.002) apresentado no XLIII Congresso Brasileiro de Fitopatologia, 2010, Cuiabá.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 13. | | BASSO, M. F.; FAJARDO, T. V. M.; SANTOS, H. P. dos; GUERRA, C. C.; AYUB, R. A.; NICKEL, O. Fisiologia foliar e qualidade enológica da uva em videiras infectadas por vírus. Tropical Plant Pathology, Brasília, v. 35, n. 6, p. 351-359, nov./dez. 2010.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 14. | | BASSO, M. F.; FAJARDO, T. V. M.; SILVA, J. C. F.; ALFENAS-ZERBINI, P.; ZERBINI, F. M. Association of a novel highly divergent monopartite circular ssDNA virus with chlorotic dwarf and dry branches in apple and pear and potential association with symptoms in grapevine. In: INTERNATIONAL GEMINIVIRUS SYMPOSIUM, 7.; INTERNATIONAL SSDNA COMPARATIVE VIROLOGY WORKSHOP, 5., 2013, Hangzhou. Program and abstracts... [S.l.: s.n., 2013]. p. 56.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 15. | | BASSO, M. F.; SILVA, J. C. F.; FAJARDO, T. V. M.; FONTES, E. P. B.; ZERBINI, F. M. A novel, highly divergent ssDNA virus identified in Brazil infecting apple, pear and grapevine. Virus Research, v. 14, n. 210, p. 27-33, July 2015.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 17. | | BASSO, M. F.; FERREIRA, P. C. G.; KOBAYASHI, A. K.; HARMON, F. G.; NEPOMUCENO, A. L.; MOLINARI, H. B. C.; GROSSI-DE-SA, M. F. MicroRNAs and new biotechnological tools for its modulation and improving stress tolerance in plants. Plant Biotechnology Journal, v. 17, p. 1482-1500, 2019.| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| 18. | | MARTINS, P. K.; MAFRA, V.; SOUZA, W. R. de; RIBEIRO, A. P.; VINECKY, F.; BASSO, M. F.; DIAS, B. B. A.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Selection of reliable reference genes for RT-qPCR analysis during developmental stages and abiotic stress in Setaria viridis. Scientific Reports, V. 6, 28348, 2016.| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | BASSO, M. F.; CONTALDI, F.; CELSO, F. lo; BARATTO, C. M.; SA, M. F. G. de; BARONE, G.; FERRANTE, A.; MARTINELLI, F. Identification and expression profile of the SMAX/SMXL family genes in chickpea and lentil provide important players of biotechnological interest involved in plant branching. Planta, v. 259, 2024. 1. Na publicação: Maria Fatima Grossi?de?Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 20. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; HENNING, L. M. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic Arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 49 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
27/02/2024 |
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Data da última atualização: |
12/03/2024 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ARAÚJO, G. L. T.; BASSO, M. F.; MORGANTE, C. V.; SA, M. F. G. de. |
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Afiliação: |
GABRIELE LOUISE TRINDADE ARAÚJO, Universidade Católica de Brasília; MARCOS FERNANDO BASSO, Universidade Católica de Brasília; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Overexpression of the GmGlb1-1 and GmEXPA-1 applied in cotton to increase tolerance to Meloidogyne incognita. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023. |
|
Páginas: |
p. 611. |
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Idioma: |
Inglês |
|
Conteúdo: |
Nematodes of the genus Meloidogyne pose a challenge to worldwide agricultural production, especially to economically relevant crops, such as cotton and soybean. They induce feeding sites on plant roots, leading to reduced crop yields due to impaired water and nutrient absorption. Current control methods are insufficient for this phytopathogen management. Chemical nematicides are highly toxic, crop rotation is inefficient for population elimination, and commercially resistant or tolerant cultivars are scarce. Thus, the development of less susceptible biotechnological cultivars for efficient nematode management is required. The GmGlb1-1 gene of Glycine max encodes a protein related to nitric oxide metabolism that accumulates in roots during nematode parasitism. Studies have shown that overexpression of GmGlb1-1 enhances plant resilience to oxidative stress and improves cellular homeostasis. In addition, the GmEXPA1 gene encodes an enzyme involved in cell wall lignification processes and, when overexpressed, promotes adaptability to adverse conditions. Independent overexpression of both genes has been associated with reduced susceptibility of Arabidopsis thaliana and Nicotiana tabacum to M. incognita. However, the combined effect of such genes in increasing plant resistance to nematodes has not yet been investigated. Here, we proposed to overexpress the GmGlb1-1 and GmEXPA1 genes in cotton to confer reduced susceptibility to M. incognita. Eight independent transgenic events were generated with a 1% transformation efficiency. Characterization of transgenic plants was performed by PCR and enzyme-linked immunosorbent assays. Five independent transformation events with high transgenic protein accumulation were selected for generation advancement. Progenies of T2 generation were inoculated with 2000 ppJ2 juveniles of M. incognita at 15 days after seed germination in an assay performed in greenhouse with 20 replicates of each event. After 90 days, the roots were evaluated for the incidence of galls using a qualitative rating scale. Two transgenic events stood out by having superior scores, indicating a reduction in the number of galls. Additional analyses are in progress to determine quantitative effects of the parasitism in transgenic plants. Biotechnology approaches offer great potential for application in commercial crop cultivars aiming to reduce plant susceptibility to root-knot nematodes and develop sustainable agriculture with less dependence on pesticides. MenosNematodes of the genus Meloidogyne pose a challenge to worldwide agricultural production, especially to economically relevant crops, such as cotton and soybean. They induce feeding sites on plant roots, leading to reduced crop yields due to impaired water and nutrient absorption. Current control methods are insufficient for this phytopathogen management. Chemical nematicides are highly toxic, crop rotation is inefficient for population elimination, and commercially resistant or tolerant cultivars are scarce. Thus, the development of less susceptible biotechnological cultivars for efficient nematode management is required. The GmGlb1-1 gene of Glycine max encodes a protein related to nitric oxide metabolism that accumulates in roots during nematode parasitism. Studies have shown that overexpression of GmGlb1-1 enhances plant resilience to oxidative stress and improves cellular homeostasis. In addition, the GmEXPA1 gene encodes an enzyme involved in cell wall lignification processes and, when overexpressed, promotes adaptability to adverse conditions. Independent overexpression of both genes has been associated with reduced susceptibility of Arabidopsis thaliana and Nicotiana tabacum to M. incognita. However, the combined effect of such genes in increasing plant resistance to nematodes has not yet been investigated. Here, we proposed to overexpress the GmGlb1-1 and GmEXPA1 genes in cotton to confer reduced susceptibility to M. incognita. Eight independent transgenic events wer... Mostrar Tudo |
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Palavras-Chave: |
Nematóides das galhas; Plantas geneticamente modificadas. |
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Thesagro: |
Algodão; Meloidogyne Incognita; Nematóide; Produção Agrícola. |
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Thesaurus NAL: |
Cotton; Genetically modified plants; Root-knot nematodes. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1162324/1/Overexpression-of-the-GmGlb1-1-and-GmEXPA-1-applied-in-cotton-to-increase-tolerance-to-Meloidogyne-incognita.pdf
|
|
Marc: |
LEADER 03410nam a2200265 a 4500
001 2162324
005 2024-03-12
008 2023 bl uuuu u00u1 u #d
100 1 $aARAÚJO, G. L. T.
245 $aOverexpression of the GmGlb1-1 and GmEXPA-1 applied in cotton to increase tolerance to Meloidogyne incognita.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF. Anais... Brasília, DF: Sociedade Brasileira de Fitopatologia$c2023
300 $ap. 611.
520 $aNematodes of the genus Meloidogyne pose a challenge to worldwide agricultural production, especially to economically relevant crops, such as cotton and soybean. They induce feeding sites on plant roots, leading to reduced crop yields due to impaired water and nutrient absorption. Current control methods are insufficient for this phytopathogen management. Chemical nematicides are highly toxic, crop rotation is inefficient for population elimination, and commercially resistant or tolerant cultivars are scarce. Thus, the development of less susceptible biotechnological cultivars for efficient nematode management is required. The GmGlb1-1 gene of Glycine max encodes a protein related to nitric oxide metabolism that accumulates in roots during nematode parasitism. Studies have shown that overexpression of GmGlb1-1 enhances plant resilience to oxidative stress and improves cellular homeostasis. In addition, the GmEXPA1 gene encodes an enzyme involved in cell wall lignification processes and, when overexpressed, promotes adaptability to adverse conditions. Independent overexpression of both genes has been associated with reduced susceptibility of Arabidopsis thaliana and Nicotiana tabacum to M. incognita. However, the combined effect of such genes in increasing plant resistance to nematodes has not yet been investigated. Here, we proposed to overexpress the GmGlb1-1 and GmEXPA1 genes in cotton to confer reduced susceptibility to M. incognita. Eight independent transgenic events were generated with a 1% transformation efficiency. Characterization of transgenic plants was performed by PCR and enzyme-linked immunosorbent assays. Five independent transformation events with high transgenic protein accumulation were selected for generation advancement. Progenies of T2 generation were inoculated with 2000 ppJ2 juveniles of M. incognita at 15 days after seed germination in an assay performed in greenhouse with 20 replicates of each event. After 90 days, the roots were evaluated for the incidence of galls using a qualitative rating scale. Two transgenic events stood out by having superior scores, indicating a reduction in the number of galls. Additional analyses are in progress to determine quantitative effects of the parasitism in transgenic plants. Biotechnology approaches offer great potential for application in commercial crop cultivars aiming to reduce plant susceptibility to root-knot nematodes and develop sustainable agriculture with less dependence on pesticides.
650 $aCotton
650 $aGenetically modified plants
650 $aRoot-knot nematodes
650 $aAlgodão
650 $aMeloidogyne Incognita
650 $aNematóide
650 $aProdução Agrícola
653 $aNematóides das galhas
653 $aPlantas geneticamente modificadas
700 1 $aBASSO, M. F.
700 1 $aMORGANTE, C. V.
700 1 $aSA, M. F. G. de
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Embrapa Semiárido (CPATSA) |
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